› 3hi2

X-ray diffraction
2Å resolution

Structure of the N-terminal domain of the E. coli antitoxin MqsA (YgiT/b3021) in complex with the E. coli toxin MqsR (YgiU/b3022)

Released:
DOI: 10.2210/pdb3hi2/pdb
PDB entry 3hi2 coloured by chain, front view.
PDB entry 3hi2 coloured by chain, side view.
PDB entry 3hi2 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Three dimensional structure of the MqsR:MqsA complex: a novel TA pair comprised of a toxin homologous to RelE and an antitoxin with unique properties.
Brown BL, Grigoriu S, Kim Y, Arruda JM, Davenport A, Wood TK, Peti W, Page R
PLoS Pathog 5 e1000706 (2009)
PMID: 20041169

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175149 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Antitoxin MqsA Chains: A, C
Molecule details ›
Chains: A, C
Length: 76 amino acids
Theoretical weight: 8.52 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: Q46864 (Residues: 1-76; Coverage: 58%)
Gene names: JW2989, b3021, mqsA, ygiT
Sequence domains: Antitoxin component of bacterial toxin-antitoxin system, MqsA
Structure domains: Ubiquitin-like (UB roll)
mRNA interferase toxin MqsR Chains: B, D
Molecule details ›
Chains: B, D
Length: 101 amino acids
Theoretical weight: 11.53 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: Q46865 (Residues: 1-98; Coverage: 100%)
Gene names: JW2990, b3022, mqsR, ygiU
Sequence domains: Motility quorum-sensing regulator, toxin of MqsA
Structure domains: YaeB-like fold

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P41212
Unit cell:
a: 63.234Å b: 63.234Å c: 194.886Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.205 0.248
Expression system: Escherichia coli

Quick links

Citations

35 review citations

Toxin-antitoxin systems in bacteria and archaea.
Yamaguchi et al. (2011)
34 more

12 mentions without citation

Three dimensional structure of the MqsR:MqsA complex: a novel TA pair comprised of a toxin homologous to RelE and an antitoxin with unique properties.
Brown et al. (2009)
11 more