› 3hi6

X-ray diffraction
2.3Å resolution

Crystal structure of intermediate affinity I domain of integrin LFA-1 with the Fab fragment of its antibody AL-57

Released:
DOI: 10.2210/pdb3hi6/pdb
PDB entry 3hi6 coloured by chain, front view.
PDB entry 3hi6 coloured by chain, side view.
PDB entry 3hi6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of activation-dependent binding of ligand-mimetic antibody AL-57 to integrin LFA-1.
Zhang H, Liu JH, Yang W, Springer T, Shimaoka M, Wang JH
Proc Natl Acad Sci U S A 106 18345-50 (2009)
PMID: 19805116

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-210793 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Integrin alpha-L Chains: A, B
Molecule details ›
Chains: A, B
Length: 180 amino acids
Theoretical weight: 20.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P20701 (Residues: 153-332; Coverage: 16%)
Gene names: CD11A, ITGAL
Sequence domains: von Willebrand factor type A domain
Structure domains: von Willebrand factor, type A domain
Heavy chain of Fab fragment of AL-57 against alpha L I domain Chains: H, X
Molecule details ›
Chains: H, X
Length: 220 amino acids
Theoretical weight: 23.77 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins
light chain of Fab fragment of AL-57 against alpha L I domain Chains: L, Y
Molecule details ›
Chains: L, Y
Length: 212 amino acids
Theoretical weight: 23.02 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand MN 2 x MN
Ligand SO4 6 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P65
Unit cell:
a: 133.78Å b: 133.78Å c: 161.05Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.173 0.17 0.226
Expression systems:
  • Escherichia coli
  • Cricetulus griseus

Quick links

Citations

8 citation in other articles

Sevoflurane binds and allosterically blocks integrin lymphocyte function-associated antigen-1.
Yuki et al. (2010)
7 more

16 mentions without citation

Updates to the Integrated Protein-Protein Interaction Benchmarks: Docking Benchmark Version 5 and Affinity Benchmark Version 2.
Vreven et al. (2015)
15 more