› 3him

X-ray diffraction
2.2Å resolution

The Crystal Structure of a Bacterial Regulatory Protein in the tetR Family from Rhodococcus RHA1 to 2.2A

Released:
DOI: 10.2210/pdb3him/pdb
PDB entry 3him coloured by chain, front view.
PDB entry 3him coloured by chain, side view.
PDB entry 3him coloured by chain, top view.
Source organism: Rhodococcus jostii
Entry authors: Stein AJ, Binkowski TA, Evdokimova E, Kagan O, Edwards A, Savchenko A, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171140 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH tetR-type domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 211 amino acids
Theoretical weight: 22.68 KDa
Source organism: Rhodococcus jostii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q0SD48 (Residues: 1-211; Coverage: 100%)
Gene name: RHA1_ro02733
Sequence domains:
Structure domains: Tetracycline Repressor, domain 2

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 62.053Å b: 63.042Å c: 94.766Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.197 0.248
Expression system: Escherichia coli

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