› 3hj1

X-ray diffraction
1.95Å resolution

Minor Editosome-Associated TUTase 1 with bound UTP

Released:
DOI: 10.2210/pdb3hj1/pdb
PDB entry 3hj1 coloured by chain, front view.
PDB entry 3hj1 coloured by chain, side view.
PDB entry 3hj1 coloured by chain, top view.
Source organism: Trypanosoma brucei
Primary publication:
Structure of the mitochondrial editosome-like complex associated TUTase 1 reveals divergent mechanisms of UTP selection and domain organization.
Stagno J, Aphasizheva I, Bruystens J, Luecke H, Aphasizhev R
J Mol Biol 399 464-75 (2010)
PMID: 20403364

Function and Biology Details

Reaction catalysed: 
UTP + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111670 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Terminal uridylyltransferase 7 Chains: A, B
Molecule details ›
Chains: A, B
Length: 387 amino acids
Theoretical weight: 44.52 KDa
Source organism: Trypanosoma brucei
Expression system: Escherichia coli
UniProt:
  • Canonical: C7AJA4 (Residues: 1-385; Coverage: 95%)
Gene name: MEAT1
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand UTP 2 x UTP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P21
Unit cell:
a: 62.873Å b: 101.999Å c: 66.124Å
α: 90° β: 111.65° γ: 90°
R-values:
R R work R free
0.206 0.204 0.246
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Uridine insertion/deletion editing in trypanosomes: a playground for RNA-guided information transfer.
Aphasizhev et al. (2011)
6 more

3 mentions without citation

Structural basis for the activity of a cytoplasmic RNA terminal uridylyl transferase.
Yates et al. (2012)
2 more