› 3hj9

X-ray diffraction
2Å resolution

Crystal structure of a putative nitroreductase (reut_a1228) from ralstonia eutropha jmp134 at 2.00 A resolution

Released:
DOI: 10.2210/pdb3hj9/pdb
PDB entry 3hj9 coloured by chain, front view.
PDB entry 3hj9 coloured by chain, side view.
PDB entry 3hj9 coloured by chain, top view.
Source organism: Cupriavidus pinatubonensis JMP134
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175235 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitroreductase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 223 amino acids
Theoretical weight: 24.85 KDa
Source organism: Cupriavidus pinatubonensis JMP134
Expression system: Escherichia coli
UniProt:
  • Canonical: Q472T4 (Residues: 1-222; Coverage: 100%)
Gene name: Reut_A1228
Sequence domains: Nitroreductase family
Structure domains: NADH Oxidase

Ligands and Environments

4 bound ligands:
Ligand FMN 2 x FMN
Ligand ACT 1 x ACT
Ligand MPD 1 x MPD
Ligand MG 1 x MG
1 modified residue:
Ligand MSE 18 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P3221
Unit cell:
a: 78.865Å b: 78.865Å c: 136.555Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.176 0.174 0.208
Expression system: Escherichia coli

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