3hjf

X-ray diffraction
3.06Å resolution

Crystal structure of T. thermophilus Argonaute E546 mutant protein complexed with DNA guide strand and 15-nt RNA target strand

Released:

Function and Biology Details

Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-116360 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
1 distinct RNA molecule
Macromolecules (3 distinct):
Protein argonaute Chain: A
Molecule details ›
Chain: A
Length: 685 amino acids
Theoretical weight: 76.74 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli
UniProt:
  • Canonical: Q746M7 (Residues: 1-685; Coverage: 100%)
Gene names: TT_P0026, ago
Sequence domains:
Structure domains:
5'-D(P*TP*GP*AP*GP*GP*TP*AP*GP*TP*AP*GP*GP*TP*TP*GP*TP*AP*TP*AP*GP*T)-3' Chain: X
Molecule details ›
Chain: X
Length: 21 nucleotides
Theoretical weight: 6.59 KDa
Source organism: Thermus thermophilus HB27
Expression system: Not provided
5'-R(*CP*AP*AP*CP*CP*UP*AP*CP*UP*AP*CP*CP*UP*CP*G)-3' Chain: Y
Molecule details ›
Chain: Y
Length: 15 nucleotides
Theoretical weight: 4.67 KDa
Source organism: Thermus thermophilus HB27
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P43212
Unit cell:
a: 111.868Å b: 111.868Å c: 177.035Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.202 0.255
Expression systems:
  • Escherichia coli
  • Not provided