› 3hjk

X-ray diffraction
2Å resolution

2.0 Angstrom Structure of the Ile74Val Variant of Vivid (VVD).

Released:
DOI: 10.2210/pdb3hjk/pdb
PDB entry 3hjk coloured by chain, front view.
PDB entry 3hjk coloured by chain, side view.
PDB entry 3hjk coloured by chain, top view.
Source organism: Neurospora crassa
Primary publication:
Mechanism-based tuning of a LOV domain photoreceptor.
Zoltowski BD, Vaccaro B, Crane BR
Nat Chem Biol 5 827-34 (2009)
PMID: 19718042

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189904 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PAS domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 154 amino acids
Theoretical weight: 17.5 KDa
Source organism: Neurospora crassa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9C3Y6 (Residues: 37-186; Coverage: 81%)
Gene names: G17A4.050, vvd
Sequence domains: PAS domain
Structure domains: PAS domain

Ligands and Environments

1 bound ligand:
Ligand FAD 2 x FAD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2
Spacegroup: P21
Unit cell:
a: 35.78Å b: 80.3Å c: 56.9Å
α: 90° β: 94.76° γ: 90°
R-values:
R R work R free
0.261 0.244 0.261
Expression system: Escherichia coli

Quick links

Citations

51 review citations

Function, structure and mechanism of bacterial photosensory LOV proteins.
Herrou et al. (2011)
50 more

2 mentions without citation

Mechanism-based tuning of a LOV domain photoreceptor.
Zoltowski et al. (2009)
1 more