› 3hkd

X-ray diffraction
3.7Å resolution

Tubulin-TN16 : RB3 stathmin-like domain complex

Released:
DOI: 10.2210/pdb3hkd/pdb
Model geometry
Fit model/data
PDB entry 3hkd coloured by chain, front view.
PDB entry 3hkd coloured by chain, side view.
PDB entry 3hkd coloured by chain, top view.
Source organisms:
Primary publication:
Variations in the colchicine-binding domain provide insight into the structural switch of tubulin.
Dorléans A, Gigant B, Ravelli RB, Mailliet P, Mikol V, Knossow M
Proc Natl Acad Sci U S A 106 13775-9 (2009)
PMID: 19666559

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-112169 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Tubulin alpha chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 451 amino acids
Theoretical weight: 50.19 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: D0VWZ0 (Residues: 1-451; Coverage: 100%)
Sequence domains:
Structure domains:
Tubulin beta chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 445 amino acids
Theoretical weight: 49.97 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: D0VWY9 (Residues: 1-445; Coverage: 100%)
Sequence domains:
Structure domains:
Stathmin-4 Chain: E

Ligands and Environments

4 bound ligands:
Ligand GTP 2 x GTP
Ligand MG 3 x MG
Ligand GDP 2 x GDP
Ligand N16 2 x N16
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P65
Unit cell:
a: 328.53Å b: 328.53Å c: 54.41Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.212 0.209 0.264
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 review citations

Drugs that target dynamic microtubules: a new molecular perspective.
Stanton et al. (2011)
11 more

19 mentions without citation

An overview of tubulin inhibitors that interact with the colchicine binding site.
Lu et al. (2012)
18 more