› 3hke

X-ray diffraction
3.6Å resolution

Tubulin-T138067: RB3 stathmin-like domain complex

Released:
DOI: 10.2210/pdb3hke/pdb
PDB entry 3hke coloured by chain, front view.
PDB entry 3hke coloured by chain, side view.
PDB entry 3hke coloured by chain, top view.
Source organisms:
Primary publication:
Variations in the colchicine-binding domain provide insight into the structural switch of tubulin.
Dorléans A, Gigant B, Ravelli RB, Mailliet P, Mikol V, Knossow M
Proc Natl Acad Sci U S A 106 13775-9 (2009)
PMID: 19666559

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-112169 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Tubulin alpha chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 451 amino acids
Theoretical weight: 50.19 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: D0VWZ0 (Residues: 1-451; Coverage: 100%)
Sequence domains:
Structure domains:
Tubulin beta chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 445 amino acids
Theoretical weight: 49.97 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: D0VWY9 (Residues: 1-445; Coverage: 100%)
Sequence domains:
Structure domains:
Stathmin-4 Chain: E
Molecule details ›
Chain: E
Length: 142 amino acids
Theoretical weight: 16.71 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63043 (Residues: 49-189; Coverage: 75%)
Gene name: Stmn4
Sequence domains: Stathmin family
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1

Ligands and Environments

4 bound ligands:
Ligand GTP 2 x GTP
Ligand MG 3 x MG
Ligand T13 4 x T13
Ligand GDP 2 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P65
Unit cell:
a: 326.71Å b: 326.71Å c: 54.12Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.209 0.207 0.253
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 review citations

Drugs that target dynamic microtubules: a new molecular perspective.
Stanton et al. (2011)
11 more

9 mentions without citation

An overview of tubulin inhibitors that interact with the colchicine binding site.
Lu et al. (2012)
8 more