› 3hlm

X-ray diffraction
2.5Å resolution

Crystal Structure of Mouse Mitochondrial Aspartate Aminotransferase/Kynurenine Aminotransferase IV

Released:
DOI: 10.2210/pdb3hlm/pdb
PDB entry 3hlm coloured by chain, front view.
PDB entry 3hlm coloured by chain, side view.
PDB entry 3hlm coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structure, expression, and function of kynurenine aminotransferases in human and rodent brains.
Han Q, Cai T, Tagle DA, Li J
Cell Mol Life Sci 67 353-68 (2010)
PMID: 19826765

Function and Biology Details

Reactions catalysed: 
L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate
(1a) L-kynurenine + 2-oxoglutarate = 4-(2-aminophenyl)-2,4-dioxobutanoate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138595 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aspartate aminotransferase, mitochondrial Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 401 amino acids
Theoretical weight: 44.87 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P05202 (Residues: 30-430; Coverage: 93%)
Gene names: Got-2, Got2
Sequence domains: Aminotransferase class I and II
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GOL 6 x GOL
1 modified residue:
Ligand LLP 4 x LLP

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21212
Unit cell:
a: 284.322Å b: 76.792Å c: 87.416Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.181 0.234
Expression system: Escherichia coli

Quick links

Citations

39 review citations

Kynurenines in the mammalian brain: when physiology meets pathology.
Schwarcz et al. (2012)
38 more

4 mentions without citation

Structure, expression, and function of kynurenine aminotransferases in human and rodent brains.
Han et al. (2010)
3 more