› 3hno

X-ray diffraction
2Å resolution

Crystal Structure of Pyrophosphate-dependent phosphofructokinase from Nitrosospira multiformis. Northeast Structural Genomics Consortium target id NmR42

Released:
DOI: 10.2210/pdb3hno/pdb
PDB entry 3hno coloured by chain, front view.
PDB entry 3hno coloured by chain, side view.
PDB entry 3hno coloured by chain, top view.
Source organism: Nitrosospira multiformis ATCC 25196
Entry authors: Seetharaman J, Abashidze M, Sahdev S, Janjua H, Xiao R, Ciccosanti C, Foote EL, Acton TB, Rost B, Montelione GT, Hunt JF, Tong L, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Reaction catalysed: 
Diphosphate + D-fructose 6-phosphate = phosphate + D-fructose 1,6-bisphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174137 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyrophosphate--fructose 6-phosphate 1-phosphotransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 419 amino acids
Theoretical weight: 45.11 KDa
Source organism: Nitrosospira multiformis ATCC 25196
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2YB24 (Residues: 1-419; Coverage: 100%)
Gene names: Nmul_A0740, SAMN05216403_103113, pfp
Sequence domains: Phosphofructokinase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand BR 1 x BR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: C2
Unit cell:
a: 211.895Å b: 89.322Å c: 115.462Å
α: 90° β: 119.12° γ: 90°
R-values:
R R work R free
0.216 0.216 0.241
Expression system: Escherichia coli

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