› 3hot

X-ray diffraction
3.25Å resolution

Crystal structure of the Mos1 mariner paired end complex with Mn

Released:
DOI: 10.2210/pdb3hot/pdb
PDB entry 3hot coloured by chain, front view.
PDB entry 3hot coloured by chain, side view.
PDB entry 3hot coloured by chain, top view.
Source organisms:
Primary publication:
Molecular architecture of the Mos1 paired-end complex: the structural basis of DNA transposition in a eukaryote.
Richardson JM, Colloms SD, Finnegan DJ, Walkinshaw MD
Cell 138 1096-108 (2009)
PMID: 19766564

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116365 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Mariner Mos1 transposase Chains: A, B
Molecule details ›
Chains: A, B
Length: 345 amino acids
Theoretical weight: 40.87 KDa
Source organism: Drosophila mauritiana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7JQ07 (Residues: 1-345; Coverage: 100%)
Gene name: mariner\T
Sequence domains:
Structure domains:
Mos1 NTS inverted repeat DNA Chains: C, E, G
Molecule details ›
Chains: C, E, G
Length: 25 nucleotides
Theoretical weight: 7.88 KDa
Source organism: synthetic construct
Expression system: Not provided
Mos1 TS inverted repeat DNA Chains: D, F, H
Molecule details ›
Chains: D, F, H
Length: 28 nucleotides
Theoretical weight: 8.53 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand MN 1 x MN
Ligand SO4 2 x SO4
1 modified residue:
Ligand 5IU 3 x 5IU

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P21
Unit cell:
a: 120.294Å b: 84.989Å c: 132.116Å
α: 90° β: 99.21° γ: 90°
R-values:
R R work R free
0.251 0.248 0.304
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

18 review citations

Nucleases: diversity of structure, function and mechanism.
Yang W. (2011)
17 more

9 mentions without citation

Mechanisms of DNA Transposition.
Hickman et al. (2015)
8 more