› 3hp0

X-ray diffraction
2.32Å resolution

Crystal structure of a Putative polyketide biosynthesis enoyl-CoA hydratase (pksH) from Bacillus subtilis

Released:
DOI: 10.2210/pdb3hp0/pdb
PDB entry 3hp0 coloured by chain, front view.
PDB entry 3hp0 coloured by chain, side view.
PDB entry 3hp0 coloured by chain, top view.
Source organism: Bacillus subtilis
Entry authors: Satyanarayana L, Eswaramoorthy S, Burley SK, Swaminathan S, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo trimer
Assembly name:
PDBe Complex ID:
PDB-CPX-154184 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable polyketide biosynthesis enoyl-CoA hydratase PksH Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 267 amino acids
Theoretical weight: 30.8 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P40805 (Residues: 3-259; Coverage: 99%)
Gene names: BSU17160, pksH
Sequence domains: Enoyl-CoA hydratase/isomerase
Structure domains: 2-enoyl-CoA Hydratase; Chain A, domain 1

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 48 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P32
Unit cell:
a: 80.908Å b: 80.908Å c: 218.592Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.259 0.232 0.27
Expression system: Escherichia coli

Quick links