› 3hp9

X-ray diffraction
1.6Å resolution

Crystal structure of SSB/Exonuclease I in complex with inhibitor CFAM

Released:
DOI: 10.2210/pdb3hp9/pdb
PDB entry 3hp9 coloured by chain, front view.
PDB entry 3hp9 coloured by chain, side view.
PDB entry 3hp9 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Small-molecule tools for dissecting the roles of SSB/protein interactions in genome maintenance.
Lu D, Bernstein DA, Satyshur KA, Keck JL
Proc Natl Acad Sci U S A 107 633-8 (2010)
PMID: 20018747

Function and Biology Details

Reaction catalysed: 
Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138310 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Exodeoxyribonuclease I Chain: A
Molecule details ›
Chain: A
Length: 482 amino acids
Theoretical weight: 55.51 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P04995 (Residues: 1-475; Coverage: 100%)
Gene names: JW1993, b2011, cpeA, sbcB, xonA
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand MG 1 x MG
Ligand CF1 1 x CF1
Ligand DMS 5 x DMS
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P212121
Unit cell:
a: 52.671Å b: 91.939Å c: 103.081Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.206 0.231
Expression system: Escherichia coli

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Citations

9 review citations

Architecture and conservation of the bacterial DNA replication machinery, an underexploited drug target.
Robinson et al. (2012)
8 more

1 mention without citation

Modulators of protein-protein interactions as antimicrobial agents.
Kahan et al. (2021)