› 3hr6

X-ray diffraction
1.6Å resolution

Structure of the Corynebacterium diphtheriae major pilin SpaA points to a modular pilus assembly stabilizing isopeptide bonds

Released:
DOI: 10.2210/pdb3hr6/pdb
PDB entry 3hr6 coloured by chain, front view.
PDB entry 3hr6 coloured by chain, side view.
PDB entry 3hr6 coloured by chain, top view.
Source organism: Corynebacterium diphtheriae
Primary publication:
The Corynebacterium diphtheriae shaft pilin SpaA is built of tandem Ig-like modules with stabilizing isopeptide and disulfide bonds.
Kang HJ, Paterson NG, Gaspar AH, Ton-That H, Baker EN
Proc Natl Acad Sci U S A 106 16967-71 (2009)
PMID: 19805181

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180030 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Surface-anchored fimbrial subunit Chain: A
Molecule details ›
Chain: A
Length: 436 amino acids
Theoretical weight: 46.88 KDa
Source organism: Corynebacterium diphtheriae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6NF81 (Residues: 53-486; Coverage: 87%)
Gene name: DIP2013
Sequence domains: Gram-positive pilin backbone subunit 2, Cna-B-like domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand IOD 1 x IOD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P212121
Unit cell:
a: 34.761Å b: 63.683Å c: 198.113Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.192 0.22
Expression system: Escherichia coli BL21(DE3)

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Citations

15 review citations

Architects at the bacterial surface - sortases and the assembly of pili with isopeptide bonds.
Hendrickx et al. (2011)
14 more

11 mentions without citation

A tale of two pili: assembly and function of pili in bacteria.
Kline et al. (2010)
10 more