› 3hsi

X-ray diffraction
2.2Å resolution

Crystal structure of phosphatidylserine synthase Haemophilus influenzae Rd KW20

Released:
DOI: 10.2210/pdb3hsi/pdb
PDB entry 3hsi coloured by chain, front view.
PDB entry 3hsi coloured by chain, side view.
PDB entry 3hsi coloured by chain, top view.
Source organism: Haemophilus influenzae
Entry authors: Chang C, Li H, Buck K, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
CDP-diacylglycerol + L-serine = CMP + (3-sn-phosphatidyl)-L-serine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155199 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CDP-diacylglycerol--serine O-phosphatidyltransferase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 458 amino acids
Theoretical weight: 53.65 KDa
Source organism: Haemophilus influenzae
Expression system: Escherichia coli
UniProt:
  • Canonical: P44704 (Residues: 1-455; Coverage: 100%)
Gene names: HI_0425, pssA
Sequence domains: Phospholipase D Active site motif
Structure domains: Endonuclease Chain A

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 15 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: I222
Unit cell:
a: 102.555Å b: 154.238Å c: 214.42Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.19 0.236
Expression system: Escherichia coli

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