› 3hva

X-ray diffraction
2.04Å resolution

Crystal structure of FimX GGDEF domain from Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb3hva/pdb
PDB entry 3hva coloured by chain, front view.
PDB entry 3hva coloured by chain, side view.
PDB entry 3hva coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Structural analysis of the GGDEF-EAL domain-containing c-di-GMP receptor FimX.
Navarro MV, De N, Bae N, Wang Q, Sondermann H
Structure 17 1104-16 (2009)
PMID: 19679088

Function and Biology Details

Reaction catalysed: 
2 GTP = 2 diphosphate + cyclic di-3',5'-guanylate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190963 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FimX Chains: A, B
Molecule details ›
Chains: A, B
Length: 177 amino acids
Theoretical weight: 19.13 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HUK6 (Residues: 260-431; Coverage: 25%)
Gene names: PA4959, fimX
Sequence domains: Diguanylate cyclase, GGDEF domain
Structure domains: Alpha-Beta Plaits

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P21
Unit cell:
a: 33.179Å b: 94.111Å c: 45.504Å
α: 90° β: 90.61° γ: 90°
R-values:
R R work R free
0.22 0.213 0.246
Expression system: Escherichia coli

Quick links

Citations

23 review citations

Cyclic di-GMP: the first 25 years of a universal bacterial second messenger.
Römling et al. (2013)
22 more

4 mentions without citation

Sensing the messenger: the diverse ways that bacteria signal through c-di-GMP.
Krasteva et al. (2012)
3 more