› 3hve

X-ray diffraction
2.8Å resolution

Structures of SPOP-Substrate Complexes: Insights into Molecular Architectures of BTB-Cul3 Ubiquitin Ligases: GigaxoninBTB/3-box

Released:
DOI: 10.2210/pdb3hve/pdb
PDB entry 3hve coloured by chain, front view.
PDB entry 3hve coloured by chain, side view.
PDB entry 3hve coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures of SPOP-substrate complexes: insights into molecular architectures of BTB-Cul3 ubiquitin ligases.
Zhuang M, Calabrese MF, Liu J, Waddell MB, Nourse A, Hammel M, Miller DJ, Walden H, Duda DM, Seyedin SN, Hoggard T, Harper JW, White KP, Schulman BA
Mol Cell 36 39-50 (2009)
PMID: 19818708

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190543 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Gigaxonin Chain: A
Molecule details ›
Chain: A
Length: 256 amino acids
Theoretical weight: 29.42 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9H2C0 (Residues: 1-254; Coverage: 43%)
Gene names: GAN, GAN1, KLHL16
Sequence domains:
Structure domains:
Gigaxonin Chain: B
Molecule details ›
Chain: B
Length: 256 amino acids
Theoretical weight: 27.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9H2C0 (Residues: 1-254; Coverage: 43%)
Gene names: GAN, GAN1, KLHL16
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P21
Unit cell:
a: 46.48Å b: 55.6Å c: 120.6Å
α: 90° β: 91.1° γ: 90°
R-values:
R R work R free
0.302 0.302 0.333
Expression system: Escherichia coli

Quick links

Citations

68 review citations

Molecular mechanisms of the Keap1–Nrf2 pathway in stress response and cancer evolution.
Taguchi et al. (2011)
67 more

5 mentions without citation

Expanding the genetic spectrum of giant axonal neuropathy: Two novel variants in Iranian families.
Ashrafi et al. (2023)
4 more