› 3hvu

X-ray diffraction
1.95Å resolution

1.95 Angstrom Crystal Structure of Complex of Hypoxanthine-Guanine Phosphoribosyltransferase from Bacillus anthracis with 2-(N-morpholino)ethanesulfonic acid (MES)

Released:
DOI: 10.2210/pdb3hvu/pdb
PDB entry 3hvu coloured by chain, front view.
PDB entry 3hvu coloured by chain, side view.
PDB entry 3hvu coloured by chain, top view.
Source organism: Bacillus anthracis str. 'Ames Ancestor'
Entry authors: Minasov G, Halavaty A, Shuvalova L, Dubrovska I, Winsor J, Papazisi L, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110927 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hypoxanthine phosphoribosyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 204 amino acids
Theoretical weight: 22.98 KDa
Source organism: Bacillus anthracis str. 'Ames Ancestor'
Expression system: Escherichia coli
UniProt:
  • Canonical: B9ZW32 (Residues: 1-179; Coverage: 100%)
Gene names: GBAA_0063, hpt
Sequence domains: Phosphoribosyl transferase domain
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand MES 4 x MES
Ligand NA 4 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P21
Unit cell:
a: 52.713Å b: 93.377Å c: 94.9Å
α: 90° β: 90.03° γ: 90°
R-values:
R R work R free
0.194 0.192 0.232
Expression system: Escherichia coli

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