› 3hwb

X-ray diffraction
3Å resolution

Cation selective pathway of OmpF porin revealed by anomalous diffraction

Released:
DOI: 10.2210/pdb3hwb/pdb
PDB entry 3hwb coloured by chain, front view.
PDB entry 3hwb coloured by chain, side view.
PDB entry 3hwb coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Cation-selective pathway of OmpF porin revealed by anomalous X-ray diffraction.
Dhakshnamoorthy B, Raychaudhury S, Blachowicz L, Roux B
J Mol Biol 396 293-300 (2010)
PMID: 19932117

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo trimer (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-136399 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Outer membrane porin F Chains: A, B
Molecule details ›
Chains: A, B
Length: 362 amino acids
Theoretical weight: 39.37 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P02931 (Residues: 1-362; Coverage: 100%)
Gene names: JW0912, b0929, cmlB, coa, cry, ompF, tolF
Sequence domains: Gram-negative porin
Structure domains: Porin

Ligands and Environments

2 bound ligands:
Ligand RB 19 x RB
Ligand P6G 2 x P6G
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P63
Unit cell:
a: 117.192Å b: 117.192Å c: 116.885Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.231 0.231 0.285
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Molecular Dynamics Simulation for All.
Hollingsworth et al. (2018)
2 more

5 mentions without citation

The structural biology of β-barrel membrane proteins: a summary of recent reports.
Fairman et al. (2011)
4 more