› 3hxk

X-ray diffraction
3.2Å resolution

Crystal Structure of a sugar hydrolase (YeeB) from Lactococcus lactis, Northeast Structural Genomics Consortium Target KR108

Released:
DOI: 10.2210/pdb3hxk/pdb
PDB entry 3hxk coloured by chain, front view.
PDB entry 3hxk coloured by chain, side view.
PDB entry 3hxk coloured by chain, top view.
Source organism: Lactococcus lactis subsp. lactis
Entry authors: Forouhar F, Su M, Seetharaman J, Mao M, Xiao R, Ciccosanti C, Foote EL, Maglaqui M, Zhao L, Everett JK, Nair R, Acton TB, Rost B, Montelione GT, Tong L, Hunt JF, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189971 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
BD-FAE-like domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 276 amino acids
Theoretical weight: 32.03 KDa
Source organism: Lactococcus lactis subsp. lactis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9CID4 (Residues: 1-268; Coverage: 100%)
Gene names: L42784, yeeB
Sequence domains: BD-FAE
Structure domains: alpha/beta hydrolase

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: C2221
Unit cell:
a: 68.391Å b: 150.425Å c: 218.859Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.258 0.256 0.296
Expression system: Escherichia coli BL21(DE3)

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