› 3hxp

X-ray diffraction
3.2Å resolution

Crystal structure of the FhuD fold-family BSU3320, a periplasmic binding protein component of a Fep/Fec-like ferrichrome ABC transporter from Bacillus subtilis. Northeast Structural Genomics Consortium Target SR577

Released:
DOI: 10.2210/pdb3hxp/pdb
PDB entry 3hxp coloured by chain, front view.
PDB entry 3hxp coloured by chain, side view.
PDB entry 3hxp coloured by chain, top view.
Source organism: Bacillus subtilis
Entry authors: Forouhar F, Neely H, Seetharaman J, Fang F, Xiao R, Cunningham K, Ma L, Chen CX, Everett JK, Nair R, Acton TB, Rost B, Montelione GT, Tong L, Hunt JF, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153440 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Iron(3+)-hydroxamate-binding protein FhuD Chain: A
Molecule details ›
Chain: A
Length: 300 amino acids
Theoretical weight: 33.37 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P37580 (Residues: 25-315; Coverage: 100%)
Gene names: BSU33320, fhuD
Sequence domains: Periplasmic binding protein
Structure domains: Nitrogenase molybdenum iron protein domain

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P21212
Unit cell:
a: 71.453Å b: 126.955Å c: 38.61Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.222 0.272
Expression system: Escherichia coli BL21(DE3)

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