› 3hxx

X-ray diffraction
2.11Å resolution

Crystal Structure of catalytic fragment of E. coli AlaRS in complex with AMPPCP

Released:
DOI: 10.2210/pdb3hxx/pdb
PDB entry 3hxx coloured by chain, front view.
PDB entry 3hxx coloured by chain, side view.
PDB entry 3hxx coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Paradox of mistranslation of serine for alanine caused by AlaRS recognition dilemma.
Guo M, Chong YE, Shapiro R, Beebe K, Yang XL, Schimmel P
Nature 462 808-12 (2009)
PMID: 20010690

Function and Biology Details

Reaction catalysed: 
ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133716 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alanine--tRNA ligase Chain: A
Molecule details ›
Chain: A
Length: 441 amino acids
Theoretical weight: 49.92 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P00957 (Residues: 2-442; Coverage: 50%)
Gene names: JW2667, alaS, b2697, lovB
Sequence domains: tRNA synthetases class II (A)
Structure domains: Bira Bifunctional Protein; Domain 2

Ligands and Environments

2 bound ligands:
Ligand ACP 1 x ACP
Ligand MG 3 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P212121
Unit cell:
a: 43.823Å b: 111.393Å c: 118.538Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.176 0.234
Expression system: Escherichia coli

Quick links

Citations

13 review citations

Emerging roles of tRNA in adaptive translation, signalling dynamics and disease.
Kirchner et al. (2015)
12 more

1 mention without citation

Paradox of mistranslation of serine for alanine caused by AlaRS recognition dilemma.
Guo et al. (2009)