› 3hzq

X-ray diffraction
3.82Å resolution

Structure of a tetrameric MscL in an expanded intermediate state

Released:
DOI: 10.2210/pdb3hzq/pdb
PDB entry 3hzq coloured by chain, front view.
PDB entry 3hzq coloured by chain, side view.
PDB entry 3hzq coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus MW2
Primary publication:
Structure of a tetrameric MscL in an expanded intermediate state.
Liu Z, Gandhi CS, Rees DC
Nature 461 120-4 (2009)
PMID: 19701184

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159508 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Large-conductance mechanosensitive channel Chain: A
Molecule details ›
Chain: A
Length: 114 amino acids
Theoretical weight: 12.78 KDa
Source organism: Staphylococcus aureus subsp. aureus MW2
Expression system: Escherichia coli
UniProt:
  • Canonical: P68806 (Residues: 1-94; Coverage: 78%)
Gene names: MW1235, mscL
Sequence domains: Large-conductance mechanosensitive channel, MscL
Structure domains: Large-conductance mechanosensitive channel, MscL; domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: I432
Unit cell:
a: 138.589Å b: 138.589Å c: 138.589Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.292 0.292 0.312
Expression system: Escherichia coli

Quick links

Citations

23 review citations

Mechanosensitive channels in microbes.
Kung et al. (2010)
22 more

10 mentions without citation

From membrane tension to channel gating: A principal energy transfer mechanism for mechanosensitive channels.
Zhang et al. (2016)
9 more