› 3i02

X-ray diffraction
2.6Å resolution

Crystal structure of S54-10 antibody in complex with antigen Kdo(2.4)Kdo(2.4)Kdo

Released:
DOI: 10.2210/pdb3i02/pdb
PDB entry 3i02 coloured by chain, front view.
PDB entry 3i02 coloured by chain, side view.
PDB entry 3i02 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Antibodies raised against chlamydial lipopolysaccharide antigens reveal convergence in germline gene usage and differential epitope recognition.
Brooks CL, Müller-Loennies S, Borisova SN, Brade L, Kosma P, Hirama T, Mackenzie CR, Brade H, Evans SV
Biochemistry 49 570-81 (2010)
PMID: 20000757

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-213278 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Immunoglobulin light chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 219 amino acids
Theoretical weight: 24.33 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
Immunoglobulin heavy chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 224 amino acids
Theoretical weight: 24.29 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: KDA, KDO
Ligand KDA 2 x KDA
Ligand KDO 4 x KDO
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-002+
Spacegroup: P21
Unit cell:
a: 64.98Å b: 53.36Å c: 142.23Å
α: 90° β: 102.13° γ: 90°
R-values:
R R work R free
0.214 0.211 0.268

Quick links

Citations

2 review citations

Antibody recognition of carbohydrate epitopes†.
Haji-Ghassemi et al. (2015)
1 more

2 mentions without citation

Prediction of carbohydrate binding sites on protein surfaces with 3-dimensional probability density distributions of interacting atoms.
Tsai et al. (2012)
1 more