› 3i0u

X-ray diffraction
2.7Å resolution

Structure of the type III effector/phosphothreonine lyase OspF from Shigella flexneri

Released:
DOI: 10.2210/pdb3i0u/pdb
PDB entry 3i0u coloured by chain, front view.
PDB entry 3i0u coloured by chain, side view.
PDB entry 3i0u coloured by chain, top view.
Source organism: Shigella flexneri
Entry authors: Singer AU, Skarina T, Nocek B, Gordon R, Lam R, Kagan O, Edwards AM, Joachimiak A, Chirgadze NY, Anderson WF, Savchenko A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186625 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphothreonine lyase OspF Chains: A, B
Molecule details ›
Chains: A, B
Length: 218 amino acids
Theoretical weight: 25.42 KDa
Source organism: Shigella flexneri
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8VSP9 (Residues: 23-239; Coverage: 91%)
Gene names: CP0010, SFLP011, mkaD, ospF, pWR501_0013
Sequence domains: Salmonella virulence-associated 28kDa protein
Structure domains: phosphothreonine lyase

Ligands and Environments

1 bound ligand:
Ligand MPD 1 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P41212
Unit cell:
a: 62.125Å b: 62.125Å c: 239.586Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.252 0.25 0.287
Expression system: Escherichia coli

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