› 3i1j

X-ray diffraction
1.9Å resolution

Structure of a putative short chain dehydrogenase from Pseudomonas syringae

Released:
DOI: 10.2210/pdb3i1j/pdb
PDB entry 3i1j coloured by chain, front view.
PDB entry 3i1j coloured by chain, side view.
PDB entry 3i1j coloured by chain, top view.
Source organism: Pseudomonas syringae pv. tomato
Entry authors: Singer AU, Evdokimova E, Kudritska M, Edwards AM, Joachimiak A, Savchenko A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183421 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Oxidoreductase, short chain dehydrogenase/reductase family Chains: A, B
Molecule details ›
Chains: A, B
Length: 247 amino acids
Theoretical weight: 26.72 KDa
Source organism: Pseudomonas syringae pv. tomato
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q885U1 (Residues: 1-246; Coverage: 100%)
Gene name: PSPTO_1740
Sequence domains: short chain dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

4 bound ligands:
Ligand EDO 9 x EDO
Ligand ACT 1 x ACT
Ligand NA 4 x NA
Ligand CL 1 x CL
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P212121
Unit cell:
a: 47.253Å b: 71.599Å c: 131.086Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.166 0.224
Expression system: Escherichia coli BL21(DE3)

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