› 3i1k

X-ray diffraction
2.1Å resolution

Structure of porcine torovirus Hemagglutinin-Esterase

Released:
DOI: 10.2210/pdb3i1k/pdb
PDB entry 3i1k coloured by chain, front view.
PDB entry 3i1k coloured by chain, side view.
PDB entry 3i1k coloured by chain, top view.
Source organism: Porcine torovirus
Primary publication:
Structural basis for ligand and substrate recognition by torovirus hemagglutinin esterases.
Langereis MA, Zeng Q, Gerwig GJ, Frey B, von Itzstein M, Kamerling JP, de Groot RJ, Huizinga EG
Proc Natl Acad Sci U S A 106 15897-902 (2009)
PMID: 19721004

Function and Biology Details

Reaction catalysed: 
N-acetyl-O-acetylneuraminate + H(2)O = N-acetylneuraminate + acetate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180693 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
sialate O-acetylesterase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 377 amino acids
Theoretical weight: 41.96 KDa
Source organism: Porcine torovirus
Expression system: Homo sapiens
UniProt:
  • Canonical: Q70KP4 (Residues: 24-393; Coverage: 87%)
Gene name: HE
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand ACY 3 x ACY
Ligand NAG 16 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: C2
Unit cell:
a: 156.307Å b: 104.528Å c: 97.473Å
α: 90° β: 95.79° γ: 90°
R-values:
R R work R free
0.173 0.171 0.205
Expression system: Homo sapiens

Quick links

Citations

11 review citations

Viral quasispecies evolution.
Domingo et al. (2012)
10 more

4 mentions without citation

Structural basis for ligand and substrate recognition by torovirus hemagglutinin esterases.
Langereis et al. (2009)
3 more