› 3i4h

X-ray diffraction
2.25Å resolution

Crystal structure of Cas6 in Pyrococcus furiosus

Released:
DOI: 10.2210/pdb3i4h/pdb
PDB entry 3i4h coloured by chain, front view.
PDB entry 3i4h coloured by chain, side view.
PDB entry 3i4h coloured by chain, top view.
Source organism: Pyrococcus furiosus
Primary publication:
Cas6 is an endoribonuclease that generates guide RNAs for invader defense in prokaryotes.
Carte J, Wang R, Li H, Terns RM, Terns MP
Genes Dev 22 3489-96 (2008)
PMID: 19141480

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186415 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CRISPR-associated endoribonuclease Cas6 Chain: X
Molecule details ›
Chain: X
Length: 273 amino acids
Theoretical weight: 31.79 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8U1S4 (Residues: 2-264; Coverage: 100%)
Gene names: PF1131, cas6
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 18-ID
Spacegroup: P3221
Unit cell:
a: 84.745Å b: 84.745Å c: 81.679Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.23 0.226 0.289
Expression system: Escherichia coli

Quick links

Citations

103 review citations

CRISPR/Cas, the immune system of bacteria and archaea.
Horvath et al. (2010)
102 more

12 mentions without citation

Evolution and classification of the CRISPR-Cas systems.
Makarova et al. (2011)
11 more