› 3i4i

X-ray diffraction
1.89Å resolution

Crystal structure of a prokaryotic beta-1,3-1,4-glucanase (lichenase) derived from a mouse hindgut metagenome

Released:
DOI: 10.2210/pdb3i4i/pdb
PDB entry 3i4i coloured by chain, front view.
PDB entry 3i4i coloured by chain, side view.
PDB entry 3i4i coloured by chain, top view.
Source organism: uncultured murine large bowel bacterium BAC 14
Entry authors: Nakatani Y, Nalder TD, Tannock GW, Cutfield JF, Jack RW, Carne A

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176763 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-glucanase Chains: A, B
Molecule details ›
Chains: A, B
Length: 234 amino acids
Theoretical weight: 26.91 KDa
Source organism: uncultured murine large bowel bacterium BAC 14
Expression system: Escherichia coli
UniProt:
  • Canonical: Q58WW0 (Residues: 28-243; Coverage: 89%)
Gene name: bgl14A1
Sequence domains: Glycosyl hydrolases family 16
Structure domains: Jelly Rolls

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 79.367Å b: 79.804Å c: 85.47Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.17 0.215
Expression system: Escherichia coli

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