› 3i4t

X-ray diffraction
2.49Å resolution

Crystal structure of putative diphthine synthase from Entamoeba histolytica

Released:
DOI: 10.2210/pdb3i4t/pdb
PDB entry 3i4t coloured by chain, front view.
PDB entry 3i4t coloured by chain, side view.
PDB entry 3i4t coloured by chain, top view.
Source organism: Entamoeba histolytica
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
4 S-adenosyl-L-methionine + 2-((3S)-3-carboxy-3-aminopropyl)-L-histidine-[translation elongation factor 2] = 4 S-adenosyl-L-homocysteine + diphthine methyl ester-[translation elongation factor 2]
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111314 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
diphthine methyl ester synthase Chain: A
Molecule details ›
Chain: A
Length: 292 amino acids
Theoretical weight: 33.33 KDa
Source organism: Entamoeba histolytica
Expression system: Escherichia coli
UniProt:
  • Canonical: C4LWZ7 (Residues: 1-271; Coverage: 100%)
Gene name: EHI_127240
Sequence domains: Tetrapyrrole (Corrin/Porphyrin) Methylases
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P4322
Unit cell:
a: 84.894Å b: 84.894Å c: 89.402Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.224 0.273
Expression system: Escherichia coli

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