› 3i4u

X-ray diffraction
2.1Å resolution

Crystal Structure Analysis of a helicase associated domain

Released:
DOI: 10.2210/pdb3i4u/pdb
PDB entry 3i4u coloured by chain, front view.
PDB entry 3i4u coloured by chain, side view.
PDB entry 3i4u coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural analysis of the C-terminal domain of the spliceosomal helicase Prp22.
Kudlinzki D, Schmitt A, Christian H, Ficner R
Biol Chem 393 1131-40 (2012)
PMID: 23096351

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172032 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent RNA helicase DHX8 Chain: A
Molecule details ›
Chain: A
Length: 270 amino acids
Theoretical weight: 31.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q14562 (Residues: 950-1183; Coverage: 19%)
Gene names: DDX8, DHX8
Sequence domains:
Structure domains: Four Helix Bundle (Hemerythrin (Met), subunit A)

Ligands and Environments

2 bound ligands:
Ligand BR 3 x BR
Ligand GOL 7 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A, BESSY BEAMLINE 14.2
Spacegroup: P41212
Unit cell:
a: 78.854Å b: 78.854Å c: 88.793Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.193 0.245
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

From keys to bulldozers: expanding roles for winged helix domains in nucleic-acid-binding proteins.
Harami et al. (2013)
4 more

2 mentions without citation

Roles of individual domains in the function of DHX29, an essential factor required for translation of structured mammalian mRNAs.
Dhote et al. (2012)
1 more