› 3i5d

X-ray diffraction
3.46Å resolution

Crystal structure of the ATP-gated P2X4 ion channel in the closed, apo state at 3.5 Angstroms (R3)

Released:
DOI: 10.2210/pdb3i5d/pdb
PDB entry 3i5d coloured by chain, front view.
PDB entry 3i5d coloured by chain, side view.
PDB entry 3i5d coloured by chain, top view.
Source organism: Danio rerio
Primary publication:
Crystal structure of the ATP-gated P2X(4) ion channel in the closed state.
Kawate T, Michel JC, Birdsong WT, Gouaux E
Nature 460 592-8 (2009)
PMID: 19641588

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-243204 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
P2X purinoceptor Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 356 amino acids
Theoretical weight: 40.19 KDa
Source organism: Danio rerio
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q6NYR1 (Residues: 28-381; Coverage: 91%)
Gene name: p2rx4a
Sequence domains: ATP P2X receptor
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand NAG 10 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: R3
Unit cell:
a: 234.91Å b: 234.91Å c: 138.433Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.277 0.276 0.289
Expression system: Spodoptera frugiperda

Quick links

Citations

146 review citations

Physiology of microglia.
Kettenmann et al. (2011)
145 more

22 mentions without citation

New structure enlivens interest in P2X receptors.
Browne et al. (2010)
21 more