› 3i6t

X-ray diffraction
1.9Å resolution

Crystal structure of muconate cycloisomerase from Jannaschia sp.

Released:
DOI: 10.2210/pdb3i6t/pdb
PDB entry 3i6t coloured by chain, front view.
PDB entry 3i6t coloured by chain, side view.
PDB entry 3i6t coloured by chain, top view.
Source organism: Jannaschia sp. CCS1
Entry authors: Bonanno JB, Freeman J, Bain KT, Do J, Romero R, Sauder JM, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
(+)-muconolactone = cis,cis-muconate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173335 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mandelate racemase/muconate lactonizing enzyme C-terminal domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 381 amino acids
Theoretical weight: 41.32 KDa
Source organism: Jannaschia sp. CCS1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q28SI7 (Residues: 2-371; Coverage: 100%)
Gene name: Jann_1408
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand K 4 x K
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P4
Unit cell:
a: 134.196Å b: 134.196Å c: 89.74Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.169 0.205
Expression system: Escherichia coli

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