› 3i76

X-ray diffraction
2Å resolution

The crystal structure of the orthorhombic form of the putative HAD-hydrolase YfnB from Bacillus subtilis bound to magnesium reveals interdomain movement

Released:
DOI: 10.2210/pdb3i76/pdb
PDB entry 3i76 coloured by chain, front view.
PDB entry 3i76 coloured by chain, side view.
PDB entry 3i76 coloured by chain, top view.
Source organism: Bacillus subtilis
Entry authors: Bonanno JB, Dickey M, Bain KT, Tang BK, Romero R, Sauder JM, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126514 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative HAD-hydrolase YfnB Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 244 amino acids
Theoretical weight: 28.52 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: O06480 (Residues: 2-234; Coverage: 99%)
Gene names: BSU07330, yfnB
Sequence domains: haloacid dehalogenase-like hydrolase
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 4 x MG
Ligand CL 4 x CL
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P212121
Unit cell:
a: 43.685Å b: 67.964Å c: 271.404Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.175 0.219
Expression system: Escherichia coli

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