› 3i83

X-ray diffraction
1.9Å resolution

Crystal structure of 2-dehydropantoate 2-reductase from Methylococcus capsulatus

Released:
DOI: 10.2210/pdb3i83/pdb
PDB entry 3i83 coloured by chain, front view.
PDB entry 3i83 coloured by chain, side view.
PDB entry 3i83 coloured by chain, top view.
Source organism: Methylococcus capsulatus
Entry authors: Bonanno JB, Gilmore M, Bain KT, Chang S, Sampathkumar P, Sauder JM, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
(R)-pantoate + NADP(+) = 2-dehydropantoate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178785 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-dehydropantoate 2-reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 320 amino acids
Theoretical weight: 34.45 KDa
Source organism: Methylococcus capsulatus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q604L6 (Residues: 2-310; Coverage: 99%)
Gene names: MCA2523, panE
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ACY 1 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P212121
Unit cell:
a: 75.851Å b: 79.179Å c: 100.626Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.192 0.227
Expression system: Escherichia coli BL21(DE3)

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