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3i9t

X-ray diffraction
2.15Å resolution

Crystal structure of the rat heme oxygenase (HO-1) in complex with heme binding dithiothreitol (DTT)

Released:
Model geometry
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Function and Biology Details

Reaction catalysed:
[heme b + 3 reduced [NADPH--hemoprotein reductase] + 3 O2 = biliverdinIXalpha + CO + Fe(2+) + 3 oxidized [NADPH--hemoprotein reductase] + 3 H2O+ H(+).]

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139216 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heme oxygenase 1 Chain: A
Molecule details ›
Chain: A
Length: 263 amino acids
Theoretical weight: 30.3 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P06762 (Residues: 1-261; Coverage: 90%)
Gene name: Hmox1
Sequence domains: Heme oxygenase
Structure domains: Heme oxygenase-like

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
1 bound ligand:
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P3221
Unit cell:
a: 65.739Å b: 65.739Å c: 120.663Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.165 0.162 0.209
Expression system: Escherichia coli