› 3iag

X-ray diffraction
2Å resolution

CSL (RBP-Jk) bound to HES-1 nonconsensus site

Released:
DOI: 10.2210/pdb3iag/pdb
PDB entry 3iag coloured by chain, front view.
PDB entry 3iag coloured by chain, side view.
PDB entry 3iag coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Thermodynamic and structural insights into CSL-DNA complexes.
Friedmann DR, Kovall RA
Protein Sci 19 34-46 (2010)
PMID: 19866488

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116399 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Recombining binding protein suppressor of hairless Chain: C
Molecule details ›
Chain: C
Length: 422 amino acids
Theoretical weight: 47.87 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P31266 (Residues: 53-474; Coverage: 80%)
Gene names: Igkjrb1, Igkrsbp, Rbpj, Rbpsuh
Sequence domains:
Structure domains:
5'-D(*AP*AP*TP*CP*TP*TP*TP*CP*AP*CP*AP*CP*GP*AP*T)-3' Chain: A
Molecule details ›
Chain: A
Length: 15 nucleotides
Theoretical weight: 4.53 KDa
Source organism: Mus musculus
Expression system: Not provided
5'-D(*TP*TP*AP*TP*CP*GP*TP*GP*TP*GP*AP*AP*AP*GP*A)-3' Chain: B
Molecule details ›
Chain: B
Length: 15 nucleotides
Theoretical weight: 4.65 KDa
Source organism: Mus musculus
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand EDO 6 x EDO
Ligand XYL 1 x XYL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21212
Unit cell:
a: 63.451Å b: 93.137Å c: 112.569Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.197 0.243
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

5 review citations

Applications of isothermal titration calorimetry in pure and applied research--survey of the literature from 2010.
Ghai et al. (2012)
4 more

11 mentions without citation

Protein detection by nanopores equipped with aptamers.
Rotem et al. (2012)
10 more