› 3ian

X-ray diffraction
1.75Å resolution

Crystal structure of a chitinase from Lactococcus lactis subsp. lactis

Released:
DOI: 10.2210/pdb3ian/pdb
PDB entry 3ian coloured by chain, front view.
PDB entry 3ian coloured by chain, side view.
PDB entry 3ian coloured by chain, top view.
Source organism: Lactococcus lactis subsp. lactis
Entry authors: Bonanno JB, Rutter M, Bain KT, Miller S, Ozyurt S, Sauder JM, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189954 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
chitinase Chain: A
Molecule details ›
Chain: A
Length: 321 amino acids
Theoretical weight: 35.92 KDa
Source organism: Lactococcus lactis subsp. lactis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9CE95 (Residues: 42-351; Coverage: 63%)
Gene names: L9964, chiA
Sequence domains: Glycosyl hydrolases family 18
Structure domains: Glycosidases

Ligands and Environments

2 bound ligands:
Ligand NA 1 x NA
Ligand EDO 10 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P43212
Unit cell:
a: 74.771Å b: 74.771Å c: 156.196Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.169 0.192
Expression system: Escherichia coli BL21(DE3)

Quick links