› 3iax

X-ray diffraction
2.6Å resolution

The crystal structure of the TolB box of Colicin A in complex with TolB reveals important differences in the recruitment of the common TolB translocation portal used by group A colicins

Released:
DOI: 10.2210/pdb3iax/pdb
PDB entry 3iax coloured by chain, front view.
PDB entry 3iax coloured by chain, side view.
PDB entry 3iax coloured by chain, top view.
Source organisms:
Primary publication:
The crystal structure of the TolB box of colicin A in complex with TolB reveals important differences in the recruitment of the common TolB translocation portal used by group A colicins.
Zhang Y, Li C, Vankemmelbeke MN, Bardelang P, Paoli M, Penfold CN, James R
Mol Microbiol 75 623-36 (2010)
PMID: 19627502

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137861 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tol-Pal system protein TolB Chain: A
Molecule details ›
Chain: A
Length: 438 amino acids
Theoretical weight: 47.07 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A855 (Residues: 1-430; Coverage: 100%)
Gene names: JW5100, b0740, tolB
Sequence domains:
Structure domains:
Colicin-A Chain: B
Molecule details ›
Chain: B
Length: 115 amino acids
Theoretical weight: 11.97 KDa
Source organism: Citrobacter freundii
Expression system: Escherichia coli
UniProt:
  • Canonical: P04480 (Residues: 1-107; Coverage: 18%)
Gene name: caa

Ligands and Environments

3 bound ligands:
Ligand NA 1 x NA
Ligand CA 1 x CA
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P21
Unit cell:
a: 61.955Å b: 40.174Å c: 80.868Å
α: 90° β: 97.18° γ: 90°
R-values:
R R work R free
0.193 0.19 0.257
Expression system: Escherichia coli

Quick links

Citations

8 review citations

TonB or not TonB: is that the question?
Krewulak et al. (2011)
7 more

2 mentions without citation

The crystal structure of the TolB box of colicin A in complex with TolB reveals important differences in the recruitment of the common TolB translocation portal used by group A colicins.
Zhang et al. (2010)
1 more