› 3ic6

X-ray diffraction
2.59Å resolution

Crystal structure of putative methylase family protein from Neisseria gonorrhoeae

Released:
DOI: 10.2210/pdb3ic6/pdb
PDB entry 3ic6 coloured by chain, front view.
PDB entry 3ic6 coloured by chain, side view.
PDB entry 3ic6 coloured by chain, top view.
Source organism: Neisseria gonorrhoeae FA 1090
Entry authors: Chang C, Marshall N, Cobb G, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177075 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA/rRNA methyltransferase SpoU type domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 223 amino acids
Theoretical weight: 24.69 KDa
Source organism: Neisseria gonorrhoeae FA 1090
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5F8U0 (Residues: 1-220; Coverage: 81%)
Gene name: NGO_0670
Sequence domains: SpoU rRNA Methylase family
Structure domains: Alpha/beta knot

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 9 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P6522
Unit cell:
a: 73.123Å b: 73.123Å c: 193.315Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.21 0.209 0.244
Expression system: Escherichia coli BL21(DE3)

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