› 3ica

X-ray diffraction
2.44Å resolution

The crystal structure of the beta subunit of a phenylalanyl-tRNA synthetase from Porphyromonas gingivalis W83

Released:
DOI: 10.2210/pdb3ica/pdb
PDB entry 3ica coloured by chain, front view.
PDB entry 3ica coloured by chain, side view.
PDB entry 3ica coloured by chain, top view.
Source organism: Porphyromonas gingivalis
Entry authors: Fan Y, Sather A, Keigher L, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181785 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phenylalanine--tRNA ligase beta subunit Chains: A, B
Molecule details ›
Chains: A, B
Length: 213 amino acids
Theoretical weight: 24.53 KDa
Source organism: Porphyromonas gingivalis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7MXR4 (Residues: 503-712; Coverage: 26%)
Gene names: PG_0099, pheT
Sequence domains: Phenylalanyl tRNA synthetase beta chain CLM domain
Structure domains: Bira Bifunctional Protein; Domain 2

Ligands and Environments

2 bound ligands:
Ligand GOL 11 x GOL
Ligand TAM 1 x TAM
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P43212
Unit cell:
a: 59.725Å b: 59.725Å c: 256.266Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.202 0.255
Expression system: Escherichia coli

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