› 3icl

X-ray diffraction
2Å resolution

X-Ray Structure of Protein (EAL/GGDEF domain protein) from M.capsulatus, Northeast Structural Genomics Consortium Target McR174C

Released:
DOI: 10.2210/pdb3icl/pdb
PDB entry 3icl coloured by chain, front view.
PDB entry 3icl coloured by chain, side view.
PDB entry 3icl coloured by chain, top view.
Source organism: Methylococcus capsulatus
Entry authors: Kuzin A, Chen Y, Seetharaman J, Mao M, Xiao R, Ciccosanti C, Foote EL, Wang H, Everett JK, Nair R, Acton TB, Rost B, Montelione GT, Tong L, Hunt JF, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178839 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
EAL/GGDEF domain protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 171 amino acids
Theoretical weight: 18.94 KDa
Source organism: Methylococcus capsulatus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q60BX6 (Residues: 60-223; Coverage: 34%)
Gene name: MCA0337
Sequence domains: Diguanylate cyclase, GGDEF domain
Structure domains: Alpha-Beta Plaits

Ligands and Environments

1 bound ligand:
Ligand SO4 8 x SO4
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P212121
Unit cell:
a: 40.672Å b: 75.95Å c: 95.196Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.208 0.222
Expression system: Escherichia coli

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