› 3ics

X-ray diffraction
1.94Å resolution

Crystal structure of partially reduced Bacillus anthracis CoADR-RHD

Released:
DOI: 10.2210/pdb3ics/pdb
PDB entry 3ics coloured by chain, front view.
PDB entry 3ics coloured by chain, side view.
PDB entry 3ics coloured by chain, top view.
Source organism: Bacillus anthracis str. Ames
Primary publication:
Crystal structure and catalytic properties of Bacillus anthracis CoADR-RHD: implications for flavin-linked sulfur trafficking.
Wallen JR, Mallett TC, Boles W, Parsonage D, Furdui CM, Karplus PA, Claiborne A
Biochemistry 48 9650-67 (2009)
PMID: 19725515

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105688 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Rhodanese domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 588 amino acids
Theoretical weight: 66.01 KDa
Source organism: Bacillus anthracis str. Ames
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A6L7H7X4 (Residues: 2-554; Coverage: 100%)
Gene name: GBAA_0774
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand FAD 2 x FAD
Ligand COA 2 x COA
Ligand ADP 1 x ADP
1 modified residue:
Ligand MSE 40 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: P21
Unit cell:
a: 67.37Å b: 110.47Å c: 80.02Å
α: 90° β: 101.87° γ: 90°
R-values:
R R work R free
0.185 0.183 0.228
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Glutathione analogs in prokaryotes.
Fahey RC. (2013)
3 more

3 mentions without citation

FAD/NADH Dependent Oxidoreductases: From Different Amino Acid Sequences to Similar Protein Shapes for Playing an Ancient Function.
Trisolini et al. (2019)
2 more