› 3icy

X-ray diffraction
2.68Å resolution

The crystal structure of sensory box histidine kinase/response regulator domain from Chlorobium tepidum TLS

Released:
DOI: 10.2210/pdb3icy/pdb
PDB entry 3icy coloured by chain, front view.
PDB entry 3icy coloured by chain, side view.
PDB entry 3icy coloured by chain, top view.
Source organism: Chlorobaculum tepidum
Entry authors: Wu R, Shonda C, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185101 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
histidine kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 118 amino acids
Theoretical weight: 13.4 KDa
Source organism: Chlorobaculum tepidum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8KAU2 (Residues: 160-274; Coverage: 17%)
Gene name: CT2060
Sequence domains: PAS fold
Structure domains: PAS domain

Ligands and Environments

1 bound ligand:
Ligand GOL 4 x GOL
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: C2
Unit cell:
a: 106.728Å b: 32.483Å c: 93.755Å
α: 90° β: 114.67° γ: 90°
R-values:
R R work R free
0.171 0.167 0.248
Expression system: Escherichia coli

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