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X-ray diffraction
1.8Å resolution

Structure of Glycosomal Glyceraldehyde-3-Phosphate Dehydrogenase from Trypanosoma cruzi in complex with the irreversible iodoacetamide inhibitor

Released:
DOI: 10.2210/pdb3ids/pdb
PDB entry 3ids coloured by chain, front view.
PDB entry 3ids coloured by chain, side view.
PDB entry 3ids coloured by chain, top view.
Source organism: Trypanosoma cruzi
Entry authors: Balliano TL, Guido RVC, Andricopulo AD, Oliva G

Function and Biology Details

Reaction catalysed: 
D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149657 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glyceraldehyde-3-phosphate dehydrogenase, glycosomal Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 359 amino acids
Theoretical weight: 39.11 KDa
Source organism: Trypanosoma cruzi
Expression system: Escherichia coli
UniProt:
  • Canonical: P22513 (Residues: 1-359; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 1 x NAD
2 bound ligands:
Ligand ACM 3 x ACM
Ligand GOL 6 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE W01B-MX2
Spacegroup: P21
Unit cell:
a: 83.121Å b: 85.388Å c: 105.66Å
α: 90° β: 96.42° γ: 90°
R-values:
R R work R free
0.213 0.2 0.24
Expression system: Escherichia coli

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