› 3ie7

X-ray diffraction
1.6Å resolution

The crystal structure of phosphofructokinase (lin2199) from Listeria innocua in complex with ATP at 1.6A

Released:
DOI: 10.2210/pdb3ie7/pdb
PDB entry 3ie7 coloured by chain, front view.
PDB entry 3ie7 coloured by chain, side view.
PDB entry 3ie7 coloured by chain, top view.
Source organism: Listeria innocua
Entry authors: Satyanarayana L, Burley SK, Swaminathan S, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
ATP + D-tagatose 6-phosphate = ADP + D-tagatose 1,6-bisphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187835 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tagatose-6-phosphate kinase Chain: A
Molecule details ›
Chain: A
Length: 320 amino acids
Theoretical weight: 35.34 KDa
Source organism: Listeria innocua
Expression system: Escherichia coli
UniProt:
  • Canonical: Q929S5 (Residues: 2-310; Coverage: 100%)
Gene name: lin2199
Sequence domains: pfkB family carbohydrate kinase
Structure domains: UDP-N-acetylmuramoyl-L-alanine:D-glutamate ligase

Ligands and Environments

3 bound ligands:
Ligand ATP 1 x ATP
Ligand MG 3 x MG
Ligand GOL 1 x GOL
1 modified residue:
Ligand MSE 7 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: C2
Unit cell:
a: 146.108Å b: 46.244Å c: 57.762Å
α: 90° β: 102.08° γ: 90°
R-values:
R R work R free
0.219 0.219 0.239
Expression system: Escherichia coli

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