› 3iem

X-ray diffraction
2.5Å resolution

Crystal Structure of TTHA0252 from Thermus thermophilus HB8 complexed with RNA analog

Released:
DOI: 10.2210/pdb3iem/pdb
PDB entry 3iem coloured by chain, front view.
PDB entry 3iem coloured by chain, side view.
PDB entry 3iem coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Entry authors: Ishikawa H, Nakagawa N, Kuramitus S, Yokoyama S, Masui R

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero pentamer
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178190 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Ribonuclease TTHA0252 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 431 amino acids
Theoretical weight: 47.12 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SLP1 (Residues: 1-431; Coverage: 100%)
Gene name: TTHA0252
Sequence domains:
Structure domains:
RNA (5'-R(*(SSU)P*(SSU)P*(SSU)P*(SSU)P*(SSU)P*(SSU))-3') Chains: G, H, I, J, K, L, M, N, O, P
Molecule details ›
Chains: G, H, I, J, K, L, M, N, O, P
Length: 6 nucleotides
Theoretical weight: 1.89 KDa
Source organism: Thermus thermophilus HB8
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand SO4 59 x SO4
Ligand ZN 8 x ZN
Ligand FLC 1 x FLC
1 modified residue:
Ligand SSU 60 x SSU

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44B2
Spacegroup: C2
Unit cell:
a: 144.694Å b: 146.441Å c: 119.975Å
α: 90° β: 110.37° γ: 90°
R-values:
R R work R free
0.213 0.213 0.257
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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