› 3if0

X-ray diffraction
2.2Å resolution

Crystal Structure of the Nanoarchaeum equitans tRNA splicing endonuclease structural subunit

Released:
DOI: 10.2210/pdb3if0/pdb
PDB entry 3if0 coloured by chain, front view.
PDB entry 3if0 coloured by chain, side view.
PDB entry 3if0 coloured by chain, top view.
Source organism: Nanoarchaeum equitans
Primary publication:
Crystal structure and assembly of the functional Nanoarchaeum equitans tRNA splicing endonuclease.
Mitchell M, Xue S, Erdman R, Randau L, Söll D, Li H
Nucleic Acids Res 37 5793-802 (2009)
PMID: 19578064

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181084 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA intron endonuclease catalytic domain-containing protein Chain: X
Molecule details ›
Chain: X
Length: 153 amino acids
Theoretical weight: 18.45 KDa
Source organism: Nanoarchaeum equitans
Expression system: Escherichia coli
UniProt:
  • Canonical: Q74MS9 (Residues: 1-153; Coverage: 100%)
Gene name: NEQ261
Sequence domains: tRNA intron endonuclease, catalytic C-terminal domain
Structure domains: Trna Endonuclease; Chain: A, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P3221
Unit cell:
a: 95.434Å b: 95.434Å c: 47.791Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.25 0.248 0.277
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Diversity and roles of (t)RNA ligases.
Popow et al. (2012)
6 more

2 mentions without citation

Recent insights into the structure, function, and regulation of the eukaryotic transfer RNA splicing endonuclease complex.
Hayne et al. (2022)
1 more