› 3ifx

Hybrid

Crystal structure of the Spin-labeled KcsA mutant V48R1

Released:
DOI: 10.2210/pdb3ifx/pdb
PDB entry 3ifx coloured by chain, front view.
PDB entry 3ifx coloured by chain, side view.
PDB entry 3ifx coloured by chain, top view.
Source organism: Streptomyces lividans
Primary publication:
Electron spin-echo envelope modulation (ESEEM) reveals water and phosphate interactions with the KcsA potassium channel.
Cieslak JA, Focia PJ, Gross A
Biochemistry 49 1486-94 (2010)
PMID: 20092291

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assemblies composition:
homo tetramer (preferred)
homo octamer
Assembly name:
PDBe Complex ID:
PDB-CPX-141094 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
pH-gated potassium channel KcsA Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 129 amino acids
Theoretical weight: 13.94 KDa
Source organism: Streptomyces lividans
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A334 (Residues: 1-123; Coverage: 77%)
Gene names: kcsA, skc1
Sequence domains: Ion channel
Structure domains: Helix Hairpins

Ligands and Environments

3 bound ligands:
Ligand K 2 x K
Ligand MTN 4 x MTN
Ligand TBA 1 x TBA
No modified residues

Experiments and Validation Details

Experimental Method: EPR, X-ray diffraction
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: C2
Unit cell:
a: 130.97Å b: 76.63Å c: 112.97Å
α: 90° β: 125.83° γ: 90°
R-values:
R R work R free
0.273 0.271 0.302
Expression system: Escherichia coli

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Citations

4 review citations

Use of electron paramagnetic resonance to solve biochemical problems.
Sahu et al. (2013)
3 more

5 mentions without citation

Assessing the Role of Lipids in the Molecular Mechanism of Membrane Proteins.
Jodaitis et al. (2021)
4 more